Supported software versions file extension applied biosystems 7900ht fast real. It is designed to support several pcrrelated chemistries. The abi qpcr software control software comes equipped with simple stepbystep wizard instruction to help you set up your assways. Keck center 7900 ht realtime quantitative pcr practical operating guide. Synthesis of 1st cdna strand cdna oligo dt or random hexamer 3. Consequently, if more gene transcript is in the target sample kidney or. Read and understand the material safety data sheets msdss provided by the manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste. Abi prism 7900ht sequence detection system user guide. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item. The taqman array is also available for use on the 7900ht fast realtime pcr system and is used to analyze tens to hundreds of targets with multiple samples. Assaysondemand products and assaysbydesign sm service.
The applied biosystems 7900ht fast realtime pcr system is a fully automated, high throughput system that allows users to run realtime pcr assays in different modes including absolute quantification, relative quantification and allelic discrimination. Used applied biosystems thermo 7900ht pcr system lab. We can connect to the 7900ht via the sds software, however, upon pressing the openclose button, the door opens and the tray arm oscillates but not extends. Applied biosystems 7900ht fast realtime pcr system site preparation. Applied biosystems provides pre formulated, readytouse, qualitytested, 5 nuclease assays for use with the 7900ht fast realtime pcr system see table below. Box with cords and cables for applied biosystems 7900ht fast realtime pcr system.
Keck center 7900 ht realtime quantitative pcr practical. I think the 7900ht machine is what applied biosystems use. Sometimes this is efortful because removing this by hand takes some knowhow related to windows program uninstallation. Applied biosystems perkin elmer geneamp pcr system 9700 thermal. The side access door was opened and closed, extending and retracting the 96well plate arm. Applied biosystems 7900ht fast realtime pcr system user. The automation accessory combined with 384well plate and 7900ht micro fluidic card capability make the 7900ht system ideally suited to meet the highthroughput requirements of todays drug discovery process. This is a secondgeneration sequence detection system designed for automated, highthroughput detection of fluorescent pcrrelated chemistries. Connect with a variety of sellers on labx and buy today. Applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide pn 4351684, with any changes as noted below. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems real fast. Create a new plate document within the sds software by following the next steps. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr. Reagents and disposables a complete line of reagents and consumables is available for use with the abi prism 7900ht sequence detection system.
Some of the chemicals used with applied biosystems instruments and protocols are potentially hazardous and can cause injury, illness, or death. Applied biosystems 7900ht sequence detection system. Absolute quantitation using standard curve assay getting started guide for the 7900ht system vii applied biosystems 7900ht fast realtime pcr system maintenance and troubleshooting guide pn 4365542 applied biosystems 7900ht fast realtime pcr system site preparation guide pn 4351923. Fast realtime pcr system and sds enterprice database system is used for fluorescentbased pcr chemistries to provide quantitative detection of nucleic acid sequences. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. Select the data collection tab, then verify the default settings. Abi prism 7000 sequence detection systems relative. Pdf use of applied biosystems 7900ht sequence detection.
The applied biosystems realtime pcr systems chemistry guide provides an easy. Abi applied biosystems 7900ht fast realtime pcr system. Applied biosystems microamp optical 384well reaction. Performing maintenance and thermo fisher scientific. Applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide. The technician paired the 7900ht pcr with the pc workstation and sds software by starting a new experiment in the software. The applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide describes how to prepare, maintain, and troubleshoot the applied biosystems 7900ht fast realtime pcr system instrument, zymark twister microplate handler, and sds enterprise database. The sds software features two types of runs to support a. Applied biosystems 7900ht fast realtime pcr system absolute. The patch improves the usability of the rq manager software and resolves several software issues, including a critical issue that affects 7900ht fast systems running.
Applied biosystems 7900ht fast realtime pcr system software download. Installing the fast 96well block and fast 96well plate adapter. The abi fast 7900ht realtime pcr instrument is an industry workhorse, in use for many years and still at the forefront in many laboratories around the world. Applied biosystems 7900ht fast realtime pcr system. Sybr concentration should not be used for instance sybr select from applied biosystems. Sometimes, computer users decide to erase this program. Applied biosystems abi prism 7900ht sequence detection system system had an integrated thermal cycling block sold with original books, software disks, cables and computer. Applied biosystems stepone and steponeplus realtime pcr system. The sds software sets gene expression levels for the calibrator sample to 1 log of zero. The 7900ht contains the hardware used for thermal cycling and detection of fluorescent chemistries. View online or download applied biosystems 7900ht user manual. Select the ramp rate tab, then verify the default settings. The abi 7300 is great quantifying nucleic acid sequences without compromising data. Certified scientific instruments has a team of oem trained engineers who rebuild these instruments.
Applied biosystems 7300 real time pcr system andbio. Applied biosystems 7300 real time pcr system the abi 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. Software system software included at no additional cost. This stateoftheart system was engineered to support applications such as snp genotyping and gene expression. Thermo abi 7900ht pcr w stacker made in 2009 applied biosystems part number 4330966 thermo. Shop online for a wide selection of applied biosystems microamp optical 384well reaction plate with barcode designed to provide unmatched temperature accuracy and uniformity. Refurbished applied biosystems 7300 real time pcr system w.
Find pricing on new and used applied biosystems 7900hts and other lab products. Applied biosystems solid ez bead emulsifier fully tested free. Complete the plate document setup as described in the applied biosystems 7900ht fast realtime pcr system and sds enterprise. User bulletin applied biosystems realtime pcr systems july 2007 subject. Other machines similar to applied biosystems 7500, fast realtime pcr. Operating the 7900ht instrument using the sds software. Applied biosystems geneamp 9700 pcr thermal cycler 96 well block. When you install the fast 96well block, make sure that you also install the fast 96well plate adapter.
This small but robust pcr takes up less space than the applied biosystems 7900ht. User bulletin applied biosystems 7900ht fast realtime pcr system 112008 subject. The system includes a builtin thermal cycler, a laser to induce fluorescence, ccd chargecoupled device det. In addition, the applied biosystems 7300 and abi 7500 sds software gives you the ability to sort, graph and export data to extenisions such as microsoft powerpoint. The applied biosystems analysis software can import and analyze data generated by any of the supported instruments listed in the following table. Applied biosystems 7900ht fast realtime pcr system thermo. The applied biosystems 7500 fast dx realtime pcr instrument with sds software is a realtime nucleic acid amplification and fivecolor fluorescence detection system available for. Manufacturer specifications 7900ht, applied biosystems abi please note. Abi applied biosystems 7900ht fast realtime pcr sequence. The applied biosystems 7900ht fast realtime pcr system employs the automation controller software for automated operation of the 7900ht instrument.
Read and understand the material safety data sheets msdss provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials. The software versions listed in the table represent only those tested for use with the applied biosystems software. Applied biosystems 7900ht pcr sequence detection system w. Applied biosystems 7900ht fast realtime pcr system and sds. Applied biosystems 7500 fast dx realtime pcr instrument. Use of applied biosystems 7900ht sequence detection system and taqman assay for detection of quinoloneresistant neisseria gonorrhoeae. Applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide pn 4351684. All hardware, including ccd, heated cover assembly. Configuring the the software coordinates the action of the 7900ht instrument, the bar code reader, software and the plate handler while acquiring and saving raw data during each run.
Used applied biosystems 4330966 thermo abi 7900ht pcr. Applied biosystems 7900ht user manual pdf download. The abi prism 7900ht sequence detection systemis a highthroughput realtime pcr system that detects and quantitates nucleic acid sequences. Without the calibration kits and dyes, the technicians cannot perform actual testing pcr.
Applied biosystems 7900ht fast realtime pcr system and sds enterprise. Applied biosystems 7900ht fast realtime pcr system and. Relative quantitation rq algorithms in applied biosystems realtime pcr systems software. Performing calibration and verification runs maintaining the 7900ht instrument hardware maintaining the computer and software maintaining the automation accessory troubleshooting chemistry and assay runs flags and filtering.
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